急需高手帮忙翻译一下中文摘要的英文翻译，非常感谢！！中文如下

Question

目的：了解甲状腺激素受体β△基因在大鼠不同组织中的分布特点，了解实时荧光定量PCR的原理及使用方法。熟悉采用实时荧光定量PCR的方法分析结果。方法：TRβ△基因在多种组织中均有表达，其中肝脏、脑、骨胳肌、心脏、肾脏表达最多。提取大鼠肝脏、脑、骨胳肌、心脏、肾脏等多处组织的mRNA, 以提取的mRNA为模板逆转录合成cDNA第一链。并以之为模板PCR扩增成双链cDNA。根据转录出的目的基因设计两条引物。采用实时荧光定量PCR的方法进行定量分析。结果：1.提取组织总RNA，OD260/OD280均介于1.7-2.0之间，OD260/230介于1.5-1.9之间。经甲醛变性琼脂糖凝胶电泳，28S与18S的密度比值约2。2.琼脂糖凝胶电泳鉴定PCR扩增结果：1）GAPDH PCR产物的琼脂糖凝胶电泳鉴定结果显示：在预期的约160bp左右有目的扩增产物带。2）TRβΔ PCR产物的琼脂糖凝胶电泳鉴定结果显示：经PCR扩增出的DNA分子量大小为176bp的目的条带。3）重组克隆质粒pGEM-基因的鉴定结果：以EcoRⅠ对重组质粒进行酶切，2％琼脂糖凝胶电泳检测酶切结果，可以看到在3000bp左右的质粒片段，以及与插入序列大小相符的另一条DNA的条带。目的基因及管家基因定量测定，其扩增曲线和溶解曲线未见杂峰，同时融解曲主峰对应的Tm值与扩增产物的理论Tm相近。结论：研究结果显示TRβ△基因于mRNA水平的表达有一定的空间分布特点：肝，脑，脾，肾组织中均有该基因表达，且脾中的表达量明显高于其他组织。

Answer 1

Understands the thyroxine acceptor β△ gene in big mouse different organization distributed characteristic, the understanding real-time fluorescence quota PCR principle and the application method. Uses real-time fluorescence quota PCR familiar the method analysis result. Method: TRβ△ the gene has the expression in many kinds of organizations, the liver, the brain, the skeleton muscle, the heart, the kidney expression are most. Withdraws the big mouse liver, the brain, the skeleton muscle, the heart, the kidney and so on many place organization's mRNA, withdraws mRNA is the template counter duplication synthesizes the cDNA first chain. And increases double strand cDNA take it as template PCR. According to the goal gene which copies designs two to direct the thing. Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis. Finally: 1. the extraction organizes total RNA, OD260/OD280 to be situated between 1.7-2.0, OD260/230 is situated between 1.5-1.9. After formaldehyde denatured agarose gel electrophoresis, 28S and 18S density ratio approximately 2. 2. the agarose gel electrophoresis appraises PCR to increase the result: 1) GAPDH the PCR product's agarose gel electrophoresis appraisal result showed: Has the goal about anticipated 160bp approximately to increase the product belt. 2) TRβΔ the PCR product's agarose gel electrophoresis appraisal result showed: The DNA molecular weight size which increases after PCR is the 176bp goal banding. 3) reorganizes the clone material particle pGEM- gene the appraisal result: By EcoRⅠCarries on the enzyme to the reorganization material particle to cut, 2% agarose gel electrophoresis examination enzyme cuts the result, may see about the 3000bp material particle fragment, as well as with insertion sequence size match case another DNA banding. The goal gene and the housekeeping gene quantitative determination, it increases the curve and the curve of solubility has not seen the mixed peak, simultaneously melts the tune prominent peak correspondence Tm value with to increase the product theory Tm to be close. Conclusion: The findings showed that TRβ△ the gene has certain spatial distribution characteristic in the mRNA level's expression: The liver, the brain, the spleen, in the kidney organization has this gene expression, and in spleen's expression quantity is higher than other organizations obviously

Answer 2

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