急!!!请教高手帮我翻译一下药品PPM说明书的一部分,详见问题补充,万分感激

PPM™ significantly simplifies the tissue culture working procedures as follows:
1. Media containing PPM™ may be dispensed outside the laminar flow hood (LFH) exposed to the ambient air. The plates should be covered soon after agar solidification. In the event that media dispensing is done by a pump, we recommend passing autoclaved hot water through the hoses prior to and after media dispensing.
2. Heat sensitive or heat stable liquid media containing PPM™ does not need to be sterilized by Millipore filters or autoclaved provided that it will be stored in sterile containers and that the stock solutions are not contaminated. In rich media containing 200 mg/liter or more of amino acids or proteins, it is recommended to filter the media with the PPM™.
3. If working in the LFH the utensils (forceps or scalpels) do not need to be flamed. They should be periodically dipped in 70% alcohol. The LFH does not need to be certified and the work can be done as well outside the LFH on a clean surface for a period not exceeding 1 hour.
4. PPM™ is an acidic liquid solution (pH 3.8). PPM™ should be stored at 4°C. (see Safety Information below). At the recommended dose of 0.05 - 0.2% (v/v), PPM™ is added to the medium before or after autoclavation to prevent airborne and endogenous contamination at low inoculum densities.
5. PPM™ is less effective when exposed to high density of bacteria or fungi spores found regularly on seed's coat. For in vitro germination, seeds should be conventionally surface sterilized with EPA registered bleach. Therefore, in the presence of PPM™ (in the germination medium), the seeds can be rinsed under tap water in a non-sterile strainer and left to dry preferably in the LFH. If the utensil ends have touched active bacteria, fungi culture or otherwise suspected of being contaminated, they should be sterilized by autoclave or by use of an electric heating element.

PPM的™大大简化了工作程序,组织培养如下:
1。媒体载的PPM™外,则可免去层流罩暴露在空气(联丰亨)。板块应覆盖琼脂凝固后不久。在配药,媒体是由泵完成的情况下,我们建议通过软管经过蒸压热水之前和之后的媒体分配。
2。热敏感或热稳定的PPM™不含有需要由微孔过滤器消毒或灭菌提供,这将是存放在无菌容器中,而股票解决方案并不受污染液体介质。在含有200毫克/升或氨基酸或蛋白质,更是建议进行筛选的PPM™媒体丰富的媒体。
3。如果工作在联丰亨器皿(镊子或解剖刀)不需要被火烧。他们应定期用70%酒精浸泡。在联丰亨不需要申请认证的工作可以做,以及一个干净的表面上外联丰亨一段不超过1小时。
4。 PPM的™是一种酸性液体溶液(pH值3.8)。 PPM的™应贮存在4℃ (见安全信息如下)。在推荐剂量为0.05 - 0.2%(第V / V)时,PPM的™添加到中期之前或之后autoclavation接种,以防止在低空气密度和内源污染。
5。 PPM的™是不太有效时,接触到的细菌或真菌的孢子上找到种子高密度的外套定期。对于离体萌发,种子表面应常规消毒,漂白环保局登记。因此,在PPM的存在™(在发芽介质),种子可在水龙头下冲洗水在非无菌过滤器和干燥的左联丰亨最好。如果器具两端有感动活性细菌,真菌培养或以其他方式被污染嫌疑的,应当经过消毒的高压灭菌器或通过电加热元件的使用。
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第1个回答  2011-06-15
PPM ?大大简化了组织培养工作程序如下:
1。媒体含PPM ?可以允许层流罩外(LFH)暴露在空气。盘子应该投保琼脂凝固后不久。在该事件,媒体胶是由泵,我们建议通过蒸压热水通过软管在媒体前和调剂。
2。热敏感或热稳定的液体含有PPM ?不需要Millipore消毒过滤或蒸压但将会被储存在的无菌容器中,股票的解决方案不被污染。在丰富的媒体含200毫克/升或多个氨基酸或蛋白质,建议过滤媒体PPM ?
3。如果工作在LFH器皿(镊子或手术刀)不需要爆发。他们应该定期蘸上含有70%的酒精。LFH的不需要注册和工作可以通过以外的LFH放在干净的表面上,有效期不超过1小时。
4。PPM ?是一种酸性溶液(pH值3.8)。PPM ?应储存在4°C。(见安全信息如下)。在推荐剂量为0.05 - 0.2%(v / v)、PPM ?加给媒体之前或之后的autoclavation机载和内源污染预防接种剂在低密度较高。
5。PPM ?当暴露在更为有效高密度的细菌或真菌孢子发现定期在种子的外套。体外发芽、种子应与传统表面灭菌EPA注册漂白。因此,当着PPM ?(在萌发中),种子可在水龙头下冲洗水过滤器和non-sterile留给干尤其是LFH。如果异物末端摸活性细菌、真菌文化或其他被怀疑是污染,他们应该消毒或釜使用一种电热元件。
大概的意思应该懂吧本回答被网友采纳
第2个回答  2011-06-15
下载有道词典,翻译很快,因为内容太多所以我没打出来,