2.2 Stabilization experiment of peanut milk
Different stabilizers were individually added into the prepared peanut milk. The intermixture was poured into bottles, sterilized, and stored for 72 h at room temperature (23℃) . Observations were recorded on the texture and state of the peanut milk according to stratification .
23 Microorganisms used as inocula
Microorganisms used, which were from the laboratory collection of Shanghai Fishenes University, are as follows: Bifidobacterium bifidum ( Bb) , Lactobacillus acidophillus ( La) , mixture of Lactobacillus bulgaricus and Streptococcus thermophilus ( Yc) , and mixture of Bb, La. and Yc (Mx) .
2.4 Cultrire conditions for cultures
Ye La Bb Mx
10% nonfat milk powder 10% nonfat milk powder with 2% fresh carrot juice and tomato juice
Sierilizing condition : O . 7kg/cmz , 15 min
Inoculate with 5% inoculum after coojed
42℃ . 6 - 8 h 37℃ . 6 - 8 h 37℃ , 6 - 8 h. anaerobic 37℃,5- 6 h. anaerobic
The four cultures were incubated several times until coagulated
2.5 Fermentation procedure
Different types of carbohydrates were severally added to the peanut milk, inoculated with 5% inoculum . The mixtures were distributed in 250mL glass boffles and incubated at 42℃(Yc) and 37℃ (La, Bb, Mx) for 6 - 8 h. The pH value, titratable acidity, and viable cell counts were then measured .
Oligofructose at 0.5% (Raftilose , Orafti Co.,Belgium) and 0.2% yeast extract were also added to thepeanut milk for La,Bb,and Mx fermentations as described in a previous study(Chen.1998)
Peanut milk fermentation with probiotic strains
2.6 Chemical analyses
Protein:Kjeldahl nitrogen method using the general appropriciate factor6.25(Wuxi University of Light Industry. 1983)
Carbohydrate :Lane and Eynon’s method ( Wuxi University of Light Industry .1983)
Fat: Soxhlet method (Wuxi University of Light Industry . 1983)
Fiber: neutral detergent metbod ( Ni , 1992)
pH: pH meter
Titratable acidity (TA) : measured by titrating with 0.1 M NaOH for 100 mL sample
2.7 Microbiological analyses
To determine the population of viable cells in the fermented peanut milk, serial dilutions were made, and plates were cast according to the double-Jayer technique using MRS-agar. Bb was incubated under anaerobic condition for cultivation . The plates were incubated at 37℃ and 42 ℃ separately for 3 days, and typical colonies were counted .
MRS-agar medium : ( Oxoid Co . , UK)
Peptone 10 g, Lab-Lemco 8 g, Yeast extract 4 g, Dextrose 20 g, Tween 80 l ml, K2HP04 2 g, Na-acetate•3H20 5 g, NH4 citrate 2 g, MgS04•7H20 0.2 g, MnS04•4 H20 0.05 g, Agar 10 g, pH after sterilization 6.2 +/- 0.2
Effect of treatment methods on organoleptic properties of peanut milk
是电脑翻译的吗?怎么有的地方感觉不通顺呀
追答是的 因为你文章专业术语太多 不是专业人家翻译不了的.. 或者你可以请教专业学这一块的人.. 电脑翻译只能给你做个参考 只能帮你这么多了
追问谢谢,这不是我想要的。对不住了