求求翻译高手了，我翻译好多遍了，老师都说不合格，关于我的论文的

Question

镜下观察发现正常培养状态下NSCs成团生长，游离、松散细胞较少，球体较大，数量较多，而缺氧状态下的NSCs，数量较少，细胞松散，球体较小。接种后立即在相差显微镜下观察，细胞呈大量圆形亮点，边界清楚，折光性强。24h后细胞形成大量悬浮的、不规则细胞团块，部分细胞贴壁，出现较多细胞碎片，随着时间的推移，大部分悬浮细胞不断分裂生长，由含数个细胞的小细胞团，逐渐长成由几十个到近百个细胞组成大小不一的悬浮细胞球，BrdU标记免疫荧光显示NSCs成绿色荧光。MTT法检测确定缺氧时间为8h，并检测加入U0126条件下NSCs的细胞活性，6h增殖达到高峰，之后维持在这个水平，且叶酸的加入能促进增殖，叶酸缺乏抑制增殖。荧光定量PCR检测ERK1/2表达水平，复氧6h表达到达高峰，之后维持在这个水平，叶酸缺乏组低于对照组；加入U0126条件下，6h表达到达高峰，之后也维持在这个水平，U0126+叶酸缺乏组低于U0126组。Western blot法检测磷酸化ERK1/2表达水平，复氧6h表达到达高峰，之后维持在这个水平，叶酸缺乏组低于对照组；加入U0126条件下，6h表达到达高峰，之后也维持在这个水平，U0126+叶酸缺乏组低于U0126组。
就是这段，谢谢各位高手了，我后天就要打印了，谢谢谢谢

Answer 1

Microscope, observed under normal culture growth of NSCs into the group, free, loose cells less, larger sphere in larger quantities, but under hypoxic conditions NSCs, the number of small, cell loose ball smaller. Immediately after inoculation, observed under phase contrast microscope, cells were large round bright, clear boundary, refraction and strong. 24h after the formation of a large number of suspended cells, irregular cell clumps, some cells attached to the wall, there are more cell debris, over time, most of the growth of suspension cells to keep dividing by the number of cells with small cell groups, gradually grow into the tens to hundreds of cells composed of large and small ball suspension cells, BrdU labeled fluorescence signal of NSCs into the green fluorescence. MTT assay to determine ischemic time of 8h, and detection by adding U0126 cell activity under the conditions of NSCs, 6h proliferation reached a peak, then maintained at this level, and the addition of folic acid can promote the proliferation and inhibition of proliferation of folate deficiency. Quantitative PCR detection of ERK1 / 2 expression, the expression of reoxygenation 6h reached a peak, remaining at this level, folic acid deficiency group than in the control group; adding U0126 conditions, 6h expression reached its peak, after which remained at this level, U0126 + folic acid deficient group than U0126 group. Western blot detection of phosphorylated ERK1 / 2 expression levels, the peak expression of reoxygenation 6h, then maintained at this level, folic acid deficiency was lower than the control group; join U0126 conditions, 6h expression reached its peak, after which remained at this level U0126 + U0126 group of folate deficiency was lower than that.本回答被提问者和网友采纳

Answer 2

Microscopically normal training condition observed NSCs clouds, free, loose cells grow bigger, quantity is less, the ball more, and hypoxia condition, the quantity is less, the cells NSCs loose, small ball. Immediately after inoculation difference in the microscope, a large, round cell borders, refractive index. After a 24h cell formation, irregular cell suspension mass, some cells, appear more cellular debris, with the passage of time, most of the cells suspended unceasingly split growth, by a number of cells with small group of cells, gradually grew by dozens to 100 cells sizes, BrdU cells suspended NSCs showed marked immunofluorescence green fluorescence. Determine the MTT method for detecting oxygen, and testing time 8h NSCs conditions of U0126 join 6h cell activity, proliferation and maintain peak at this level, after joining and folic acid can promote the proliferation of inhibiting proliferation, folic acid. Fluorescence quantitative polymerase chain reaction (PCR) ERK1/2 expression level detection and oxygen to peak, after 6h expressed in this level, maintain the untreated group of folic acid, Join U0126 6h conditions, to express, also maintain at this level, U0126 + folic acid U0126 below lack group. Western blot ERK1 1/2 phosphorylation method to detect the expression level, express to peak oxygen 6h, maintain at this level of untreated group, folic acid, Join U0126 6h conditions, to express, also maintain at this level, U0126 + folic acid U0126 below lack group.

Answer 3

Microscope, observed under normal culture growth of NSCs into the group, free, loose cell less, larger sphere in larger quantities, but under hypoxic conditions NSCs, the number of small, cell loose ball smaller. Immediately after inoculation, observed under phase contrast microscope, cells were large round bright, clear boundary, refraction and strong. 24h after the formation of a large number of suspended cells, irregular cell clumps, some cells attached to the wall, there are more cell debris, over time, most of the growth of suspension cells to keep dividing by the number of cells with small cell groups, gradually grow into the tens to hundreds of cells composed of cells of different sizes suspended ball
BrdU labeled fluorescence signal of NSCs into the green fluorescence. MTT assay to determine ischemic time of 8h, and detection by adding U0126 cell activity under the conditions of NSCs, 6h proliferation reached a peak, then maintained at this level, and the addition of folic acid can promote the proliferation and inhibition of proliferation of folate deficiency. Quantitative PCR detection of ERK1 / 2 expression, the expression of reoxygenation 6h reached a peak, remaining at this level, folic acid deficiency group than in the control group; adding U0126 conditions, 6h expression reached its peak, after which remained at this level, U0126 + folic acid deficient group than U0126 group. Western blot, phosphorylation of ERK1 / 2 expression
, The peak expression of reoxygenation 6h, and then maintained at this level, folic acid deficiency group than in the control group; adding U0126 conditions, 6h expression reached its peak, after which remained at this level, U0126 + U0126 group was lower than folate deficiency.

Answer 4

= = 你这个...太专业了...